Comparing Responses of Ursolic Acid in Murine Macrophages Infected with Mycobacterium smegmatis and Mycobacterium avium

Mycobacterium smegmatis and avium offer an advantage in examining tuberculosis-like effects host immune defense. Therefore, the study aims to examine effect of ursolic acid (UA) on system by analyzing cytokines concentration, such as TNF-α, IL-6, IL-1β, nitrite oxide produced murine macrophages infected with avium. Femurs female C57BL/6 mice aged 6–8 weeks were used culture Bone marrow-derived (BMDM). On day 10, BMDM was using a multiplicity infection (MOI) amounting 8:1, then IL-1β analyzed ELISA Griess reagent. The results showed that UA decreased production three respective pro-inflammatory study, both For reduction nearly 65%-90% compared control. decrease IL-6 occurred from 2700 pg/ml 750 for smegmatis, while more significant those approximately 150 Moreover, reduced over 90% this result line nitrite. decreases This is preliminary but supports further role defense pathogenic non-pathogenic mycobacterial infections.